The environmental impact of improper waste cooking oil (WCO) disposal can be substantial, resulting in serious implications such as soil contamination, water pollution, energy wastage, and increased greenhouse gas emissions. To mitigate these potential impacts, the conversion of WCO into biodiesel offers an attractive alternative to fossil fuel dependency. This investigation focuses on biodiesel production via transesterification reactions, utilizing WCO collected from local food vendors. Biodiesel yield from Gino, Kings, and Mamador WCO were found to range from 55.5 to 58.1%, 55.1 to 53.9%, and 53.7 to 52.6%, respectively. Furthermore, the specific gravities of the produced biodiesel from Gino, Kings, and Mamador WCO ranged between 0.725-0.75, 0.73-0.84, and 0.71-0.80, respectively. Acid values varied from 0.51-0.52 KOH/g for Gino WCO, 0.50-0.57 KOH/g for Kings WCO, and 0.50-0.57 KOH/g for Mamador WCO. Cetane numbers were observed to range from 45.82-46.25 min for Gino WCO, 46.2-46.45 min for Kings WCO, and 46.0-46.25 min for Mamador WCO. Finally, the flashpoints ranged between 135-138℃ for Gino WCO, 137-140℃ for Kings WCO, and 137-138℃ for Mamador WCO, while cloud points hovered between 4.82-5.02℃. Significantly, all physicochemical properties of the resulting biodiesel were found to be within ASTM recommended parameters, highlighting the potential of WCO as a valuable resource for sustainable biodiesel production.