Artificial intelligence (AI) is increasingly transforming internal audit practices, yet empirical evidence concerning its adoption in public-sector auditing in Sub-Saharan Africa remains limited. This study examines whether age, gender, and educational level are associated with internal auditors’ perceptions of AI adoption in public universities in Ghana. Drawing on the Technology Acceptance Model (TAM) and the Unified Theory of Acceptance and Use of Technology (UTAUT), a quantitative cross-sectional survey was conducted among 177 internal audit staff from six public universities. Perceptions of AI adoption were assessed across four dimensions: AI adoption and accountability, fraud detection effectiveness, implementation challenges, and strategies and enablers. Overall, a favourable orientation towards AI adoption was observed, with a composite mean score of 3.614 on a five-point scale. Multiple ordinary least squares (OLS) regression indicated that the demographic model was statistically significant, F(3, 173) = 3.098, p = 0.028, although the explanatory power was modest (R² = 0.051). Age was found to be negatively associated with perceptions of AI adoption (B = −0.058, β = −0.185, p = 0.024), indicating that more favourable perceptions were reported by younger internal auditors. Educational level showed a positive association with perceptions of AI adoption and represented the strongest predictor among the demographic variables examined (B = 0.064, β = 0.220, p = 0.007), suggesting that higher levels of academic and professional education may be associated with greater readiness for AI adoption. No statistically significant association was observed for gender (p = 0.664). The findings extend empirical research on technology acceptance to public-sector internal auditing in an African context and highlight the relevance of demographic heterogeneity in understanding AI adoption readiness. In particular, age-sensitive training and opportunities for continuing academic and professional development may provide appropriate mechanisms for strengthening AI-related competencies among internal audit staff in Ghanaian public universities.